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Established and validated method for red cell depletion using followed by leukocyte concentration step with reduction of the freezable product the volume.




Efficiency in Obtaining CD34+ Cells


  Total Cells MN Cells CD34+ Cells Volume
  International Rates* 850 M 450 M 0.36% 71mL
  Cryopraxis** 970 M 480 M 0.65% 105 mL


It is very important to note that the CD 34+ cells and the MN
are the ones which better represent the potential for cell therapy



CD 34+ - Marker for stem cell hemopoiesis
MN – Mononuclear Cells
M - Millions

References:
*Honohan A et al.. Microbial contamination of cord blood stem cells. Vox Sanguinis. 2002;82:32-8.
*Bornstein, R. et al.A Modified Cord Blood Collection Method. Stem Cells.2005, 23:324-334.
**Migowski, E., et al.. Evaluation of Influence Factors on Umbilical Cord Blood Collection Contamination in Brazil.
24th Annual Meeting of the European Society for Pediatric Infectious Diseases (ESPID) Basel. Switz. 2006
© 2006 Cryopraxis Cryobiology